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antibody anti human flag rabbit polyclonal proteintech  (Proteintech)


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    Structured Review

    Proteintech antibody anti human flag rabbit polyclonal proteintech
    Antibody Anti Human Flag Rabbit Polyclonal Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+anti+human+flag+rabbit+polyclonal+proteintech/DYKDDDDK+tag+Recombinant+antibody/10__7554_slash_elife__99122-917-203-209
    Average 96 stars, based on 2143 article reviews
    antibody anti human flag rabbit polyclonal proteintech - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Construct:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    shRNA:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Negative Control:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Western Blot:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Immunohistochemistry:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Chromatin Immunoprecipitation:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Recombinant:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.

    Plasmid Preparation:

    Article Title: RUNX2 isoform II protects cancer cells from ferroptosis and apoptosis by promoting PRDX2 expression in oral squamous cell carcinoma
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) BALB/c nude mice Vital River Strain No.401 Cell line (human) CAL 27 SCC- 9 Yang et al., 2018 (PMID:29857020) Cell line (human) HEK 293T Procell Cat#CL- 0005 Transfected construct (human) RUNX2 isoform II shRNAs (shisoform II- 1 and shisoform II- 2) Vector Builder Cat#VB221206- 1024udv; VB221206- 1021ujk Lentiviral construct to transfect and express the shRNA Transfected construct (human) PRDX2 shRNAs (shPRDX- 1 and shPRDX2- 2) Vector Builder Cat#VB900064- 6571eqq; VB900064- 6578nnu Lentiviral construct to transfect and express the shRNA Transfected construct (human) Negative control shRNA (shNC) Vector Builder Cat#VB010000- 009mxc Transfected construct (human) siRNAs to isoform II (si- II- 1/si- II- 2) Sangon Biotech Transfected construct (Human) Transfected construct (human) siRNAs to HOXA10 (siHOX- 1/ siHOX- 2) GenePharma Transfected construct (Human) Transfected construct (human) siRNA to negative control (siNC) Sangon Biotech Biological sample (human) Oral squamous cell carcinoma Hospital of Stomatology, Wuhan University The adjacent normal tissues were also acquired Antibody Anti- human RUNX2 (mouse monoclonal) Santa Cruz Cat#SC- 390351; RRID:AB_2892645 WB (1:500) Antibody Anti- 4- HNE (mouse monoclonal) Abcam Cat#ab48506; RRID:AB_867452 IHC (1:600) Antibody Anti- TFRC (rabbit monoclonal) Abcam Cat#ab214039; RRID:AB_2904534 WB (1:2000) IHC (1:500) Antibody Anti- human FLAG (rabbit polyclonal) Proteintech Cat#20543- 1- AP; RRID:AB_11232216 WB (1:2000) ChIP (2 μg) Antibody Anti- human/mouse PRDX2 (rabbit polyclonal) Proteintech Cat#10545- 2- AP; RRID:AB_2168202 WB (1:2000) IHC (1:1000) Antibody Anti- human actin (mouse monoclonal) Proteintech Cat#66009- 1- lg; RRID:AB_2782959 WB (1:5000) Antibody Anti- GPX4 (rabbit monoclonal) ABclonal Cat#A11243 RRID:AB_2861533 WB (1:1000) Recombinant DNA reagent RUNX2 isoform II (plasmid) This paper FLAG- tagged isoform II Recombinant DNA reagent RUNX2 isoform I (plasmid) This paper FLAG- tagged isoform I Recombinant DNA reagent PRDX2 (plasmid) This paper FLAG- tagged PRDX2 Commercial assay or kit Total RNA Miniprep Kit Axygen Cat#AP- MN- MS- RNA- 250 Commercial assay or kit Green Taq Mix Vazyme Cat#P131- AA Commercial assay or kit Phanta Super- Fidelity DNA Polymerase Vazyme Cat#P505- d1 Huang et al. eLife 2024;13:RP99122.



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